>Dr. Ing. Stéphen HENNART – Vice President Commercial ...
The objective of this work was to investigate the mechanism of very fine grinding in a wet ball mill as a function of process parameters, i.e. rotation speed of the mill and grinding medium bead size. The ball mill used was a Dynomill and the grinding medium consisted of zirconium oxide beads. The product is a poorly water-soluble organic compound.
>Molecular Vision: Set screw homogenization of murine ...
Qiagen TissueLyser LT. Samples were collected in Safe-Lock microcentrifuge tubes (EP-022363433; Eppendorf, Hamburg, Germany). One 4.7 mm ferric ball bearing (4RJH8; Grainger, Lake Forrest, IL) was placed in each tube containing the thawed samples. The tubes were put in the tube adaptor of the Qiagen TissueLyser LT.
>Integrating a genome-wide association study with ...
The mixture was ground for 3 min in a TissueLyser ball mill (Qiagen, Germany). The extracts were centrifuged at 15,000 rpm for 5 min, and 0.5 ml of supernatant was transferred to a 1.5 ml Microfuge tube. Because the seed oil layer was heavy, this step was repeated twice and finally 0.2 ml of supernatant removed into a fresh 1.5 ml Microfuge ...
>Animals | Free Full-Text | Effect of Muscle Extract and ...
A sample of muscle tissue was suspended in ultrapure water and homogenized with frozen metal balls in a TissueLyser ball mill (Qiagen, New York, NY, USA). The homogenate was centrifuged at 1400× g for 45 min at 4 °C. The supernatant was stored at −80 °C.
>Bead Homogenizer at Thomas Scientific
BeadBlaster™ 96 Ball Mill Homogenizer. Benchmark Scientific. The BeadBlaster™ 96 is an extremely versatile bead mill homogenizer that has applications in a variety of areas, including biological research, environmental testing, and industrial settings. Adapters are available for microplates, microtubes, and 50ml tubes.
>Grinders & Homogenizers
Mills and grinders are used for to break dry or wet samples into smaller particles by grinding, crushing, or cutting. Blades are used in mills to cut up samples into finer particles. Hand-held and bench top homogenizers blend chemical or biological samples thoroughly using various methods. Sonicators are homogenizers that use sound energy to ...
>Beads - Qiagen
Product Details QIAGEN provides stainless steel beads and tungsten carbide beads for use in sample disruption with TissueLyser systems. High-speed shaking of sample material and a single bead in a Collection Microtube or 2 ml microcentrifuge tube for a few minutes allows rapid and efficient disruption of the sample.
>Method Of Grinding Of Lavender Plant | Crusher Mills, Cone ...
US Patent # 4,582,260. Method of and plant for grinding … Method of and plant for grinding pulverulent or granular materials Abstract. A vertical roller mill has a grinding table (1), rollers (3) and a separator (6) for …
>Retsch MM 300 TissueLyser Mixer Mill | eBay
Retsch MM300 TissueLyser Lab Vibration Mill Mixer provides rapid and efficient disruption of cells and tissues for the BioRobot. Processing of up to 2 x 96 samples takes as little as 2 - 4 minutes. Disruption is achieved through the beating and grinding effect of beads on the sample material as they are shaken with the sample in a variety of grinding vessels.
>DNA Extraction (Qiagen Kit)
6. Place Retch mill microfuge holder in Styro box and cool with LN 2 7. Get plant samples from step 4 and place an autoclaved stainless ball in each sample tube 8. Place samples in cooled microfuge tube holder 9. Place and secure holder containing vials into Retsch Mill, you will need cryo gloves
>DRAGON LAB, MicroPette Pipette – Proficient Lab
DRAGON LAB, MicroPette Pipette. RM 424.00. Pipette Size. Choose an option 0.5ul – 10ul 200ul – 1000ul 20ul – 200ul 2ml - 10ml. Clear. DRAGON LAB, MicroPette Pipette quantity. Add to cart. Add to quote.
>Ordering Support - Qiagen
Phone: 800-426-8157 Fax: 800-718-2056 Normal business hours are 9:00 a.m. – 7:00 p.m. (ET) on weekdays. Technical Support. Tel: 800-362-7737 Women's Health products: 800-344-3631 Fax: 661-702-3854
>TissueLyser II - Qiagen
For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used. To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight.
>GEO Accession viewer
Liver organs were ground in a Tissuelyser ball mill (Qiagen, Hilden, Germany) under cooling with liquid nitrogen. The frozen liver tissue was crushed and total RNA was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
>Modified lignite and black coal reduce ammonia ...
Before modification, the lignite sample was oven dried (70 °C), ground in a ball mill (TissueLyser II, QIAGEN), and passed through a 246 μm sieve. The BC sample was also prepared by grinding and sieving (<246 μm).
>miRNA Microarray Kits & Reagents Market Business ...
QIAGEN MilliporeSigma Geneaid Bioline ... Wet Grinding Ball Mill Market Size, Industry Share, Global Trend, Rising Demand, COVID-19 Impact, Forecast till 2027.
>Responsiveness of metallothionein and hemocyanin genes to ...
were performed applying the RNeasy Plant Mini Kit (Qiagen) with On‐Column DNA digestion (Qiagen) according to the manufacturer's instruction with following modifications: For tissue homogenization, samples were homogenized with a Precellys 24 ball mill (Bertin Corp.) in 450µl Buffer RLT+ 4.5µl 2‐mercaptoethanol (Merck). Integrity of
>12. Total RNA Extraction from Tissue of Animal
a. Ball mill homogenizer Tomy Micro Smash MS-100 [zirconia ball (5 mmφ) 3,800 rpm 120 sec] b. Rotor-Stator homogenizer [7 mmφ probe, 20,000 rpm 30 sec x 2 times] c. Pestle homogenizer for microtube with a motor [over 1 min → Add 300 µl of LRT (2-ME added) *2 and vortex for 15 sec] 17,000 x g (15,000 rpm), 3 min, RT
>Homogenization of Soy beans for DNA Extraction and Soy ...
The efficiency of seed disruption is dependent upon the type of ball mill used in the grinding process. Standard bead mills adapted to microwell plates are modeled after "paint shakers" and move the plates in a "figure-eight" motion. ... DNA Purification Using a Qiagen Genomic Column. After grinding, the soy was suspended in 10 ml of Buffer G2 ...
>Sources of variation in foliar secondary chemistry in a ...
We homogenized 100 mg of frozen leaf tissue in a ball mill (Qiagen TissueLyser, Hilden, Germany) and extracted the homogenate with 700 μL 90% methanol at pH 5 for 10 min. This solvent extracts small molecules of a wide range in …
>QuickGene RNA tissue kit S Ⅱ (RT-S2)
a. Ball mill homogenizer TOMY Micro Smash MS-100 : TOMY 2ml tube (. No.TM-625)*3 QIAGEN TissueLyser : TreffLab 2.0ml click cap (. No.96.9329.9.01) b. Rotor-Stator homogenizer 2ml tubes, etc. c. Pestle homogenizer for microtube 1.5ml tubes (attached with Pestle), etc. Ball (zirconia 5mm -) (For ball mill homogenizer only)
>Planetary Ball Mill PM 400 - RETSCH - powerful and quick ...
Planetary Ball Mills are used wherever the highest degree of fineness is required.Apart from the classical mixing and size reduction processes, the mills also meet all the technical requirements for colloidal grinding and have the energy input necessary for mechanical alloying processes.The extremely high centrifugal forces of planetary ball mills result in very high pulverization energy …
>Retsch Qiagen Tissue Lyser TissueLyzer Mixer / Grinder ...
Retsch Qiagen Tissue Lyser TissueLyzer Mixer / Grinder - Excellent. Condition is "Used". Shipped with USPS Priority Mail.
>Retsch Fluid Bed in New Jersey, USA
Used Qiagen Retsch TissueLyser II 20.747.0001 DNA Cell Disruptor (9023)R in Goleta, CA. Manufacturer: Qiagen; Qiagen Retsch TissueLyser II 20.747.0001 DNA Cell Disruptor USED (9023)R In good cosmetic condition. Powers on, digital screens and buttons respond well to input. Shakes when prompted. We have Microplates for the...
>C4 photosynthesis and the economic spectra of leaf and ...
Carbon and nitrogen concentrations of the dry leaf and root material ([C] and [N], respectively) were measured after grinding samples in a ball mill (TissueLyser 2, Qiagen) using a CN analyzer (vario EL cube, Elementar).
>A protocol for high‐throughput, untargeted forest ...
on liquid nitrogen in a ball mill (TissueLyser; QIAGEN, Hilden, Germany) and extracted the homogenate with 700 μL of 90% metha-nol : 10% water (pH 5) for 10 min. Methanol is an effective solvent for small molecules rep-resenting a wide range in polarity; mild acid-ity improves the extraction of most alkaloids.
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>Mixer Mill MM 400 - RETSCH - powerful grinding by …
This laboratory mill mixes and homogenizes up to 2 x 20 ml powders and suspensions within a few seconds. It is also perfectly suitable for the disruption of biological cells as well as for DNA/RNA and protein extraction. With its powerful performance and great flexibility, the Mixer Mill MM 400 is a unique product in the market.
>Magnetic beads, a particularly effective novel method for ...
After freezing, all samples were freeze dried, and ground to a fine powder using a ball mill (QIAGEN TissueLyser II). 2.2. DNA extraction protocols. All Ulva spp. and Saccharina latissima DNA extractions were performed on sample aliquots of 7 mg dried powder and 200 μL elution volume, to allow for comparison between DNA extraction methods.
>Host plant secondary metabolite profiling shows a complex ...
Roots and shoots were crushed using a ball mill (TissueLyser II; Qiagen, Courtaboeuf, France). Each plant part was extracted using 2 ml methanol for 10 mg of dry sample. The extraction was performed twice and extracts were pooled and dried using Speed-vac-assisted evaporation (Labconco, Kansas City, MO, USA).
>Development of a simplified and inexpensive RNA depletion ...
We further tested two commonly used commercial pDNA affinity purification kits from QIAGEN and New England Biolabs (NEB). Even though the commercial pDNA affinity extraction kits routinely included RNase A in solution, we found that significant amounts of residual bacterial RNA were presented in the pDNA preps for pMOK, a small plasmid of 3.1 kb …
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